副溶血性弧菌产耐热直接溶血毒素对黑色素瘤的抑制作用研究     

杨兰珠  李诗怡  郑雯静  李静  包哲隈  杨靖亚 《海洋科学》2022,46(8):121-129

为探讨耐热直接溶血毒素(Thermostabile direct hemolysin,TDH)在体内和体外对小鼠黑色素瘤细胞B16的抑制作用,本研究通过MTT法、克隆形成试验、凋亡试验、Caspase-8和Caspase-3的活性试验、线粒体膜电位的检测以及体内C57BL/6小鼠(Mus musculus)荷瘤实验,比较TDH作用于不同细胞的半抑制浓度(IC₅₀),评价TDH对小鼠黑色素瘤细胞B16的体内外抑制作用。结果发现:人结肠上皮细胞NCM460、人正常肝细胞LO2、人肝癌细胞SMMC-7721和小鼠黑色素瘤细胞B16在TDH处理24 h之后,细胞的半抑制质量浓度IC₅₀分别为151、118、54和48 μg/mL,正常细胞的IC₅₀高出癌细胞近2~3倍。当质量浓度低于20 μg/mL时,TDH以剂量依赖性的方式抑制B16细胞的克隆形成,6 mg/kg TDH在移植瘤模型中显著抑制体内肿瘤的生长(P<0.001)。流式细胞术和荧光试剂盒检测表明:20 μg/mL的TDH能诱导19.4%的B16细胞发生早期凋亡,并激活Caspase-8和Caspase-3,但不影响线粒体膜电位。TDH具有体内外的抗肿瘤活性,可能通过细胞表面的死亡受体介导的凋亡信号通路引起凋亡,从而发挥抗肿瘤作用。  相似文献   

Endocrine effects of methoxylated brominated diphenyl ethers in three in vitro models     

Hu W  Liu H  Sun H  Shen O  Wang X  Lam MH  Giesy JP  Zhang X  Yu H 《Marine pollution bulletin》2011,62(11):2356-2361

Methoxylated brominated diphenyl ethers (MeO-BDEs) in aquatic environments have been found to be primarily of natural origin in the marine environment and not from biotransformation of synthetic PBDEs. Two of the eight MeO-PBDEs (2′-MeO-BDE-68 and 6-MeO-BDE-47) that were detected in anchovy from the Yangtze River Delta, were natural products from marine organisms. So 2′-MeO-BDE-68 and 6-MeO-BDE-47 were chosen to study the potential to modulate androgen, estrogen, or thyroid hormone receptor- (AR, ER, ThR) mediated responses by use of reporter gene assays. 2′-MeO-BDE-68 was antiandrogenic at 50 μM, estrogenic at 10 μM and antiestrogenic at 10 and 50 μM (IC₅₀ = 4.88 μM). 2′-MeO-BDE-68 enhanced luciferase expression by 5 nM T3 at 50 μM. 6-MeO-BDE-47 exhibited potent antiandrogenicity at 1 μM and greater (IC₅₀ = 41.8 μM) and possessed estrogenic activity at 10 μM and antiestrogenic activity at 10 and 50 μM (IC₅₀ = 6.02 μM).  相似文献   

扫描电镜分析在大气颗粒物源解析中的应用   总被引：3，自引：0，他引：3  

钟宇红  房春生  邱立民  吕莉莎  张子宜  董德明  于连贵  刘辉  刘春阳  苏红石  赵静 《吉林大学学报(地球科学版)》2008,38(3):473-478

扫描电镜法作为大气颗粒物源解析的辅助方法,为源解析研究提供了定性和半定量的依据。运用扫描电镜法对吉林市采暖期和非采暖期的大气颗粒物样品以及源样品进行定性和半定量分析,将分析结果与CMB8.2化学质量平衡受体模型源解析结果进行比较,两者具有一致性。吉林市采暖期对TSP贡献较大的是扬尘和土壤风沙尘,非采暖期对TSP贡献较大的是土壤风沙尘和建筑尘。  相似文献   

Cloning and analysis of the CYP1A promoter from the atlantic killifish (Fundulus heteroclitus)     

Wade H. Powell  Hilary G. Morrison  E. Jennifer Weil  Sibel I. Karchner  Mitchell L. Sogin  John J. Stegeman  Mark E. Hahn 《Marine environmental research》2004,58(2-5):119

  相似文献   

扇贝(Chlamys farreri)多肽通过EGFR抑制受紫外线B(UVB)诱导的HaCaT细胞凋亡     

樊洁  王岩  孙秀萍  韩彦弢  王春波 《海洋与湖沼》2014,45(2):328-334

利用Hoechst 33258荧光染色法检测紫外线B(UVB)辐射诱导HaCaT细胞凋亡率。结果表明,扇贝多肽(Polypeptide from Chlamys farreri,PCF)可以剂量依赖性抑制UVB诱导的HaCaT细胞凋亡;表皮生长因子受体(EGFR)抑制剂AG1478能明显抑制UVB诱导的HaCaT细胞凋亡。采用3’-RACE法构建EGFR的cDNA片段,克隆测序检测突变位点。结果表明,UVB照射后EGFR发生碱基突变A→G,A→G,T→C,G→A,G→A;预加入5.69mmol/L的PCF,产生部分抗突变作用,1,2,3突变位点处未发生碱基的突变。  相似文献   

Estrogen receptor (ER) agonists and androgen receptor (AR) antagonists in effluents from Norwegian North Sea oil production platforms   总被引：6，自引：0，他引：6  

Tollefsen KE  Harman C  Smith A  Thomas KV 《Marine pollution bulletin》2007,54(3):277-283

The in vitro estrogen receptor (ER) agonist and androgen receptor (AR) antagonist potencies of offshore produced water effluents collected from the Norwegian Sector were determined using recombinant yeast estrogen and androgen screens. Solid phase extraction (SPE) concentrates of the effluents showed E2 agonist activities similar to those previously reported for the United Kingdom (UK) Continental Shelf (<0.1-4 ng E2 L(-1)). No activity was detected in the filtered oil droplets suggesting that produced water ER activity is primarily associated with the dissolved phase. Targeted analysis for methyl- to nonyl-substituted alkylphenol isomers show the occurrence of known ER agonists in the analysed samples. For the first time, AR antagonists were detected in both the dissolved and oil associated phase at concentrations of between 20 and 8000 microg of flutamide equivalents L(-1). The identity of the AR antagonists is unknown, however this represents a significant input into the marine environment of unknown compounds that exert a known biological effect. It is recommended that further analysis using techniques such as bioassay-directed analysis is performed to identify the compounds/groups of compounds that are responsible in order to improve the assessment of the risk posed by produced water discharges to the marine environment.  相似文献   

半胱胺对斜带石斑鱼（Epinephelus coioides）生长激素及其受体mRNA水平的影响          下载免费PDF全文

李云  蔡生力 《海洋与湖沼》2011,42(2):244-250

以海水硬骨鱼类斜带石斑鱼为研究对象,通过投喂和腹腔注射半胱胺盐酸盐（CSH）,研究半胱胺对斜带石斑鱼生长激素（GH）及其受体（GHR）mRNA水平的影响,以初步揭示半胱胺促进斜带石斑鱼生长的作用机理。结果表明,长期投喂CSH可以显著提高斜带石斑鱼的相对体重增长率。投喂和腹腔注射不同剂量的CSH均能明显促进斜带石斑鱼脑垂...  相似文献   

Modulation by Biogenic Amines for the Hemocyte Count and Prophenoloxidase Exocytosis via Receptors in Litopenaeus vannamei     

PAN Luqing  LIU Huijie  YU Jinhong 《中国海洋大学学报(英文版)》2011,10(4)

Hemocyte counts and phenoloxidase(PO)activity were examined after hemolymph being incubated in dopamine(DA),noradrenaline(NE)and serotonin(5-HT).Results showed that all the three biogenic amines(BAs)had a significant impact on total hemocyte count(THC),differential hemocyte count(DHC),and intracelluar and extracelluar phenoloxidase(PO)activity.Among these Bas,DA had the strongest effect on the above parameters,whereas 5-HT had the least effect.Preincubation with D1 receptor antagonist SCH23390,D2 receptor a...  相似文献   

基于客观赋权法的长江流域环境污染事故风险受体脆弱性时空变异特征研究   总被引：2，自引：0，他引：2  

杨小林  程书波  李义玲 《地理与地理信息科学》2015,31(2):119-124

从敏感性和恢复力两方面描述了环境风险受体脆弱性,综合考虑人群、社会经济和生态环境系统等风险受体的敏感性和恢复力,选取12个指标构建区域环境风险受体综合脆弱性评价指标体系。以长江流域为研究对象,引入"纵横向-拉开档次"法和"时序加权平均算子"法等客观赋权法探讨了流域脆弱性时空变异特征。结果表明,2000-2011年长江流域环境风险受体脆弱性呈波动下降趋势,且脆弱性空间差异较大,其中,上海、河南、江苏和西藏等省区脆弱性指数最高,陕西、重庆和云南等省市脆弱性较低。研究结果可为长江流域环境风险管理和污染事故应急管理提供依据。  相似文献   

三疣梭子蟹ftz-f1 基因的克隆及相关核受体基因在蜕皮中的功能分析     

张龙涛  吕建建  高保全  刘萍  付萍 《海洋与湖沼》2015,46(6):1390-1397

采用RACE 技术克隆三疣梭子蟹核受体fzt-f1 基因, 全长cDNA 序列1763bp.该基因3'和 5'非编码区分别为1034bp 和141bp, 开放阅读框为588bp, 推测编码195 个氨基酸, 预测分子量为 22.8kDa, 理论等电点为6.35, 命名为PTNHr 基因。同源性和系统进化分析表明, 三疣梭子蟹fzt-f1 基因与刀额新对虾同源性高达75.4%, 并且与刀额新对虾聚为一支。对核受体基因fzt-f1、EcR 和RXR 在蜕皮周期中的表达情况进行实时荧光定量PCR 分析, 结果表明, 三个核受体基因在不同蜕皮时期, 均出现表达差异, 说明这三个基因都参与蜕皮调控过程。其中, fzt-f1 和RXR 基因在不同蜕皮时期的 表达模式上出现相反的表达特征, 预测这两个基因存在相互抑制的调节关系。EcR 和RXR 基因在不 同蜕皮时期的表达模式上出现部分相似的表达特征。  相似文献